Strain Data Sheet
RBRC10694
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10694 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Grp<tm1.1(icre)Himo> |
| Former Common name | Grp-iCre |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Hisashi Mori, University of Toyama in 2019. C57BL/6-derived ES cell line RENKA was used. A pgk-Neo cassette was removed by CAG-FLPe (RIKEN BRC, RBRC01834 ). C57BL/6 genetic background. |
| Strain description | iCre was knocked in at the translation initiation site of the gastrin-releasing peptide (Grp) gene. |
| Colony maintenance | |
| References | Glucocorticoid receptor-mediated amygdalar metaplasticity underlies adaptive modulation of fear memory by stress. Inoue R, Abdou K, Hayashi-Tanaka A, Muramatsu S I, Mino K, Inokuchi K, Mori H eLife 7, e34135 (2018). 29941090 |
Health Report | |
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| Examination Date / Room / Rack | 2025/03/10Room:3-3Rack:CSentinel mouse program 2024/12/09Room:3-3Rack:CSentinel mouse program 2024/09/09Room:3-3Rack:CSentinel mouse program 2024/06/10Room:3-3Rack:CSentinel mouse program 2024/03/11Room:3-3Rack:CSentinel mouse program 2023/12/11Room:3-3Rack:CSentinel mouse program 2023/09/11Room:3-3Rack:CSentinel mouse program 2023/06/13Room:3-3Rack:CSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 18 | Frt | ||||
| Grp MGI:95833 | gastrin releasing peptide | 18 | Grp<tm1.1(icre)Himo> MGI:6201709 | targeted mutation 1.1, Hisashi Mori | |||
| iCre | phage P1 codon improved Cre (iCre) | 18 | iCre | ||||
| SV40 small t-antigen intron | 18 | ||||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Mouse Grp gene promoter, Bacteriophage P1 Improved cre recombinase gene (Genesis 32: 19-26, 2002), Simian virus 40 (SV40) small t-antigen intron, Saccharomyces cerevisiae FRT site |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. eLife 7, e34135 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application forany patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Hisashi Mori (University of Toyama) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |