Strain Data Sheet

RBRC10628

Strain Information

Image
BRC No.RBRC10628
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tgfbr2<tm1(cre/Esr1<*>)Sleep>
Former Common nameTgfbr2-CreERT2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development筑波大学・林悠、理化学研究所脳神経科学研究センター・糸原重美らにより開発。C57BL/6J由来のES細胞。C57BL/6J（日本クレア）との交配で維持。
Strain descriptionTgfbr2プロモーター制御下でCreERT2を発現するノックインマウス。Tgfbr2の元々のCDSの直後に2A-CreERT2を挿入。
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tgfbr2transforming growth factor, beta receptor II9Tgfbr2<tm1(cre/Esr1<*>)Sleep>targeted mutation, Yu Hayashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Actbchicken Beta-actin polyA9Actb

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Esr1*human Estrogen receptor9Esr1*

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide9P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase9cre

Ordering Information

供与核酸P2A (porcine teschovirus-1 2A peptide), Bacteriophage P1 Cre recombinase, Homo sapiens Estrogen receptor cDNA, chicken Beta-actin polyA, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli Neomycin resistance gene, mouse Tgfbr2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
提供条件条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。
Depositor林　悠（国立大学法人筑波大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data