Strain Data Sheet

RBRC10599

Strain Information

Image
BRC No.RBRC10599
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Slc6a4-GAP/GFP)1Ito
Former Common name5HTT-GAP43-EGFP, TCA-GFP, Sert-GAP43-EGFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentC57BL/6J由来のRP23-39F11 BAC cloneC57BL/6J（日本クレア）の♀を用いて維持、IVFに用いたオス親はN1
Strain descriptionA BAC transgenic mouse in which GAP/GFP is under the control of the Slc6a4 (solute carrier family 6 (neurotransmitter transporter, serotonin), member 4; 5Htt) gene promoter.
Colony maintenance
ReferencesNeuron, 82(2) 365-79 (2014). 24685175

Health Report

Examination Date / Room / Rack2024/03/11Room:3-5Rack:C
2023/12/11Room:3-5Rack:C
2023/09/11Room:3-5Rack:C
2023/06/13Room:3-5Rack:C
2023/05/22Room:3-5Rack:C
2023/03/13Room:3-5Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GAP43growth associated protein 43UNGAP43

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40UN

Ordering Information

供与核酸mouse serotonin transporter(5HTTP) genomic DNA, GAP43 palmitoylation signal, jellyfish EGFP cDNA, SV40 polyA, yeast FRT (flippase recombination target) site, Phage P1 loxP site derived from pBACe3.6 vector
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Mizuno et al. Neuron, 82(2) 365-79 (2014).
Depositor糸原　重美（国立研究開発法人理化学研究所）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability受注後、検査を施して提供
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data