Strain Information | |
---|---|
Image | |
BRC No. | RBRC10444 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;Cg-Gt(ROSA)26Sor<tm1(CAG-Cerulean/Arl13b,-Venus/GMNN,-Cherry/CDT1)Rmort> |
Former Common name | R26Arl13bCerulean-Fucci2aR |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Fucci2 consists of the cell cycle phase specific biosensors mCherry-Cdt1(30/120) and mVenus-hGem(1/110) fused with the Thosea asigna virus 2A peptide sequence in a single bicistronic transgene (Mort et al., 2014).Arl13bCerulean consists of the full mouse Arl13b cDNA (OTTMUST00000058920.GRCm38) and mCerulean. Arl13bCerulean-Fucci2a couples Arl13bCerulean to Fucci2a using the porcine teschovirus-1 2A (P2A) peptide on the N-terminus of Fucci2a to create a tricistronic construct. The CAG promoter incorporates the cytomegalovirus (CMV) early enhancer; the promoter, first exon and intron of the chick β-actin gene; and the beta-globin splice acceptor (Miyazaki et al., 1989). |
Strain description | Insertion by homologous combination in MESC. Targeting of CAG-floxNeo-Arl13bCerulean-Fucci2a inducible transgene to the mouse Rosa26 locus. No phenotype observed. Normal reproductive performance. |
Colony maintenance | |
References | A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Ford M J, Yeyati P L, Mali G R, Keighren M A, Waddell S H, Mjoseng H K, Douglas A T, Hall E A, Sakaue-Sawano A, Miyawaki A, Meehan R R, Boulter L, Jackson I J, Mill P, Mort R L Dev. Cell, 47, 509-523 (2018). 30458140 |
Health Report | |
---|---|
Examination Date / Room / Rack | 2024/04/22Room:3-CRack:J 2024/01/22Room:3-CRack:J 2023/10/23Room:3-CRack:J 2023/07/24Room:3-CRack:J 2023/04/24Room:3-CRack:J |
Gene | |||||||
---|---|---|---|---|---|---|---|
Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
SV40 polyA signal | 6 | ||||||
Arl13b | ADP-ribosylation factor-like 13B | 6 | Arl13b | ||||
CDT1 | human CDT1 cDNA | 6 | CDT1 | ||||
GH | Growth hormone polyA (Bovine) | 6 | GH | ||||
GMNN | geminin (human) | 6 | GMNN | ||||
Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor | targeted mutation 1 Richard Mort | |||
P2A | porcine teschovirus-1 2A peptide | 6 | P2A | ||||
T2A | Thosea asigna virus 2A peptide (insect) | 6 | T2A | ||||
Venus | Yellow Fluorescent Protein (Aequorea victoria) | 6 | Venus | ||||
loxP | phage P1 loxP | 6 | loxP | ||||
loxP | phage P1 loxP | 6 | loxP | ||||
mCerulean | Cyan Fluorescent protein (Aequorea victoria) | 6 | mCerulean | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
mCherry | Red Fluorescent Protein (Discosoma sp.) | 6 | mCherry | ||||
neo | neomycin resistance gene (E. coli) | 6 |
Phenotype | |
---|---|
Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
---|---|
Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), P1 phage loxP sites, E. coli neo, SV40 polyA, human CDT1 cDNA, Discosoma sp mCherry cDNA, T2A(Thosea asigna virus 2A peptide) , Jellyfish variant of enhanced yellow fluorescent protein (mVenus) cDNA, human GMNN cDNA, mouse Rosa26 genomic DNA, mouse Arl13b cDNA, P2A (porcine teschovirus-1 2A peptide), Aequorea victoria mCerulean cDNA, Bovine Growth hormone polyA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell, 47, 509-523 (2018).The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr Richard Mort of Lancaster University is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT. The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Ian Jackson (University of Edinburgh) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |