Strain Data Sheet

RBRC10444

Strain Information

Image
BRC No.RBRC10444
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gt(ROSA)26Sor<tm1(CAG-Cerulean/Arl13b,-Venus/GMNN,-Cherry/CDT1)Rmort>
Former Common nameR26Arl13bCerulean-Fucci2aR
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentFucci2 consists of the cell cycle phase specific biosensors mCherry-Cdt1(30/120) and mVenus-hGem(1/110) fused with the Thosea asigna virus 2A peptide sequence in a single bicistronic transgene (Mort et al., 2014).Arl13bCerulean consists of the full mouse Arl13b cDNA (OTTMUST00000058920.GRCm38) and mCerulean. Arl13bCerulean-Fucci2a couples Arl13bCerulean to Fucci2a using the porcine teschovirus-1 2A (P2A) peptide on the N-terminus of Fucci2a to create a tricistronic construct. The CAG promoter incorporates the cytomegalovirus (CMV) early enhancer; the promoter, first exon and intron of the chick β-actin gene; and the beta-globin splice acceptor (Miyazaki et al., 1989).
Strain descriptionInsertion by homologous combination in MESC. Targeting of CAG-floxNeo-Arl13bCerulean-Fucci2a inducible transgene to the mouse Rosa26 locus. No phenotype observed. Normal reproductive performance.
Colony maintenance
References
A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice.
Ford M J, Yeyati P L, Mali G R, Keighren M A, Waddell S H, Mjoseng H K, Douglas A T, Hall E A, Sakaue-Sawano A, Miyawaki A, Meehan R R, Boulter L, Jackson I J, Mill P, Mort R L
Dev. Cell, 47, 509-523 (2018). 30458140

Health Report

Examination Date / Room / Rack2024/04/22Room:3-CRack:J
2024/01/22Room:3-CRack:J
2023/10/23Room:3-CRack:J
2023/07/24Room:3-CRack:J
2023/04/24Room:3-CRack:J

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signal6
Arl13bADP-ribosylation factor-like 13B6Arl13b
CDT1human CDT1 cDNA6CDT1
GHGrowth hormone polyA (Bovine)6GH
GMNNgeminin (human)6GMNN
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sortargeted mutation 1 Richard Mort
P2Aporcine teschovirus-1 2A peptide6P2A
T2AThosea asigna virus 2A peptide (insect)6T2A
VenusYellow Fluorescent Protein (Aequorea victoria)6Venus
loxPphage P1 loxP6loxP
loxPphage P1 loxP6loxP
mCeruleanCyan Fluorescent protein (Aequorea victoria)6mCeruleanCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry
neoneomycin resistance gene (E. coli)6

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), P1 phage loxP sites, E. coli neo, SV40 polyA, human CDT1 cDNA, Discosoma sp mCherry cDNA, T2A(Thosea asigna virus 2A peptide) , Jellyfish variant of enhanced yellow fluorescent protein (mVenus) cDNA, human GMNN cDNA, mouse Rosa26 genomic DNA, mouse Arl13b cDNA, P2A (porcine teschovirus-1 2A peptide), Aequorea victoria mCerulean cDNA, Bovine Growth hormone polyA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell, 47, 509-523 (2018).The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr Richard Mort of Lancaster University is requested.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT.
The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
Nat. Biotechnol., 20, 87-90 (2002).
DepositorIan Jackson (University of Edinburgh)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. UCSD MTA
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data