Strain Data Sheet

RBRC10428

Strain Information

Image
BRC No.RBRC10428
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Xkr8<tm1.1Osa>
Former Common nameXkr8 flox/flox
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Shigekazu Ngata and Jun Suzuki at Osaka University in 2013. Bruce 4 ES cell line was used. A pair of loxP flanks Exon 3 of the Xkr8 gene. Neomycin resistance gene was removed by crossing CAG-Flp mice.
Strain descriptionXkr8 (X-linked Kx blood group related 8) floxed mice.
Colony maintenance
References
Xk-related protein 8 and CED-8 promote phosphatidylserine exposure in apoptotic cells.
Suzuki J, Denning D P, Imanishi E, Horvitz H R, Nagata S
Science, 341, 403-406 (2013). 23845944

Lupus-like autoimmune disease caused by a lack of Xkr8, a caspase-dependent phospholipid scramblase.
Kawano M, Nagata S
Proc. Natl. Acad. Sci. USA, 115(9):2132-2137 (2018). 29440417

Health Report

Examination Date / Room / Rack2023/04/10Room:3-BRack:G
2023/01/10Room:3-BRack:G

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site4Frt
Xkr8X-linked Kx blood group related 84Xkr8targeted insertion 1.1, Shigekazu Nagata
loxPphage P1 loxP4loxP
loxPphage P1 loxP4loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
  • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Xkr8 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 341, 403-406 (2013). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorShigekazu Nagata (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data