Strain Information | |
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Image | |
BRC No. | RBRC10428 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Xkr8<tm1.1Osa> |
Former Common name | Xkr8 flox/flox |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Developed by Shigekazu Ngata and Jun Suzuki at Osaka University in 2013. Bruce 4 ES cell line was used. A pair of loxP flanks Exon 3 of the Xkr8 gene. Neomycin resistance gene was removed by crossing CAG-Flp mice. |
Strain description | Xkr8 (X-linked Kx blood group related 8) floxed mice. |
Colony maintenance | |
References | Xk-related protein 8 and CED-8 promote phosphatidylserine exposure in apoptotic cells. Suzuki J, Denning D P, Imanishi E, Horvitz H R, Nagata S Science, 341, 403-406 (2013). 23845944Lupus-like autoimmune disease caused by a lack of Xkr8, a caspase-dependent phospholipid scramblase. Kawano M, Nagata S Proc. Natl. Acad. Sci. USA, 115(9):2132-2137 (2018). 29440417 |
Health Report | |
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Examination Date / Room / Rack | 2023/04/10Room:3-BRack:G 2023/01/10Room:3-BRack:G |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | 4 | Frt | ||||
Xkr8 | X-linked Kx blood group related 8 | 4 | Xkr8 | targeted insertion 1.1, Shigekazu Nagata | |||
loxP | phage P1 loxP | 4 | loxP | ||||
loxP | phage P1 loxP | 4 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Xkr8 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 341, 403-406 (2013). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Shigekazu Nagata (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |