Strain Information | |
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Image | |
BRC No. | RBRC10381 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Arf6<tm1Yaka> |
Former Common name | Arf-6 |
H-2 Haplotype | |
ES Cell line | E14K [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Yasunori Kanaho and Teruhiko Suzuki, University of Tsukuba in 2004. A neomycin resistance gene replaced a genomic region containing the translation initiation codon of the Arf6 gene. E14K ES cells dereived from 129P2/OlaHsd was used, and then mice were backcrossed to C57BL/6J over 10 generations. |
Strain description | Arf6 knockout mice. Homozygous mutants are embryonically lethal due to impaired liver development. |
Colony maintenance | |
References | Mol. Cell Biol., 26(16), 6149-56 (2006). 16880525 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)12mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK poly(A) signal, mouse Arf6 genomic DNA |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 26(16), 6149-56. (2006).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Yasunori Kanaho (University of Tsukuba) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |