Strain Data Sheet

RBRC10366

Strain Information

Image
BRC No.RBRC10366
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Dcst2<em1Nino>
Former Common nameDcst2-KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Naokazu Inoue, Fukushima Medical University in 2017. B6D2 genetic background.
Strain descriptionCd9 (CD9 antigen) mutant mice. 5 bp deletion at Exon 6. Homozygous mutant males are infertile.Dcst2<em1Nino>: 5 bp deletion at Exon 6.TAGAGAAAGGCAGAAAGTCCAGACggtcccttgagaagtagtttccctaggggtgggcaggcgggctggctcctcctgaccctcagctcccctgcctctcagtggtccagaaattctgtgtcttgccttcctacctcgaatttttcctgcgaacggtcatccgcactcgtgagtgacaccccaaccccgacctccttctgccagtgttcgcctcgtcttactgcggccactggcgctgaagtctcccagtccatccatagtgggaatgcaagagcaggatgggactttgcagggtcacccagactaatgtctaagttgatctgattgagaaactgaggcccagtgggtggaggaggagcctcattaaagccagactccagagcagaatccaggaggagcagggcctccccgctggagcataggtcacgtgggctgggaggaaccagagcgtaggtcgtggctcactcgtgatgaagtaggagccttaggccaagtctagtgctgtgtgtctggaagctgtcactgttttctagagtaggtctgggccccgggagctctagcttgtgagtcctgcaacggcagagcctggacagcccccactccgtgccctctgtcttcctaacaactgtctcggctcctgtcccctctgacctcaccggtcccagccgtgatgaaactgcttggcaagctacgtcgagagtttgagttcaacatgacagccacccactacttctctgtggacctcaattcctctcggagcctgtcccaggtggctttggacctgcaggaggctgtcagcatgaagttgtacacTGCCAGGGAGGCGCTGTCTT
Colony maintenance
ReferencesElife, 10:e66313 (2021). 33871360

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dcst2DC-STAMP domain containing 23Dcst2<em1Nino>endonuclease-mediated mutation 1, Naokazu Inoue

Ordering Information

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Elife. 2021 Apr 19;10:e66313.
DepositorNaokazu Inoue (Fukushima Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data