Strain Data Sheet

RBRC10352

Strain Information

Image
BRC No.RBRC10352
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameSTOCK Llcfc1<em2Osb>
Former Common name1700034O15Rik (Llcfc1)<-1024/wt>, SOF1
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeveloped by Taichi Noda and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2017. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed with B6D2F1. Mixed genetic background. Homozygous males are infertile.
Strain descriptionLlcfc1<em2Osb>; 1024 bp deletion at exon 1aagctccaatggaggttactgctatgcatactgatgcctgtcatcttgtgtgggagatgtgtttttccagtgacttctcatctaagtagtgactatgagaatattgttgcattagagtataggtagctatgcagatccaacagtgtaatagatatgatacttagcaacttttggaacccttttaggttgtggtatataaggtgatccattatgaccatctcagatgcctctgccatctcccacccttctgtctggcaatggaatggtataaagatCTAGAATGTAGGATATAGCCATGGCAGCGATGTCATAGGACACATAGAATCAGAGGTCCTATGGAAGAGAAGGAGGAAAAGCGATGCTGTCAAGGGGTAAGAGCTAAGAAAGGGATGGGGTGGTGTTGGTAGCTGGGGATTGGGGCCAAAGGAAGCATGGGAGAGGAGACCCATGACTTCTCTGGGCTCCCAGCTCCACAGAGCAACATTCCTGACAGCTTTGTTGCTACTGCTGCTGCTCCAAGTGAAGGGGGTGAAGACTCTGATAGTGAGTGCAAGCTTGGATGGTGACAAGAGTCAGAAAGACAAAGTGTCCTCTGAAGgtgggtagaggagagaggtagaggatggatggtgggggaggggtgctggacataagggttttcttggattcacatcaggctccagcagcagactaggtcatggttaatatggggtttgaattttggcagataactcatgttccctatcaccatagcatgaaataatagctgtaggtaaaattgtttagttttacaaagtctcttagccaggtcacagaatggggctacctacagttgtcaaaggcttctgaggaagtagagtgcctaaagagggcctctttgccttcatgtttgaagttatctctcactcctttggaggagggaactagagtaagacatggttatcacctgtgtttgagatggggctatggttgaagtgagacttgaaaagccctctagataaagcattctctcactgcgtcaaggccagtgttatgactggcagtagttacgacttatctctcaaccgttcccctcctcccctttctgataaagaatagaagttcgactggagctgcccatctctgcccttcttttatgttgtagACCAGGGTGAGGAGGAGTATGAAGAACACTTTGAGGCTTCATCAGAGGGTGAGCAGTGGCAGGAGATAGATATGGTCCAGCAAGAAGACACGATCTCCCAGGCAATAACTCTCCAGGACCACCTCCTTGACCTAGCCTTCTGCTTCAACCTGGCCAGCATCATGTTTTTTTTATGAGAGACTTTGAGGTGGAGGCAGTAGTCTGGATCCTTCATGAGGACTTAGACATCCACCTCCCCTTCCTGATTGCTTACTGACCTCCTCTCCTAAATGCCTCCTCAAAATGCACACCCTGGTCCCTGTCTCTAAGAAGTACCTGACCTTAGGGTATATCAACAGAGCTTTCCCAACTCTCATATCCACCACCTAATTGCCACCCCAACCCAGAATTTCATGTCCCG
Colony maintenance
References
Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice.
Noda T, Lu Y, Fujihara Y, Oura S, Koyano T, Kobayashi S, Matzuk M M, Ikawa M
Proc. Natl. Acad. Sci. USA, 117(21):11493-11502 (2020). 32393636

ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat.
Shunsuke Nishio, Chihiro Emori, Benjamin Wiseman, Dirk Fahrenkamp, Elisa Dioguardi, Sara Zamora-Caballero, Marcel Bokhove, Ling Han, Alena Stsiapanava, Blanca Algarra, Yonggang Lu, Mayo Kodani, Rachel E Bainbridge, Kayla M Komondor, Anne E Carlson, Michael Landreh, Daniele de Sanctis, Shigeki Yasumasu, Masahito Ikawa, Luca Jovine
Cell, 187(6):1440-1459.e24 (2024). 38490181

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Llcfc1
MGI:1923856		LLLL and CFNLAS motif containing 16Llcfc1<em2Osb>
MGI:6437831		endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Llcfc1 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 117(21):11493-11502 (2020).The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP, Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data