Strain Information | |
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Image | |
BRC No. | RBRC10339 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | STOCK Fbxl13<em1Osb> |
Former Common name | Fbxl13-30983/wt |
H-2 Haplotype | |
ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
Background strain | |
Appearance | |
Strain development | Developed by Keisuke Shimada and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2017. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed to BDF1. Mixed genetic background. |
Strain description | STOCK Fbxl13<em1Osb>; Deletin of Exon 2 to 8 of the Fbxl13CTCCCGTCCAGCCCTCTCATTTTACCCGGCCCCGGCCTGTACTGTCCTGCCCCGCCCCTCCCCGCTCTGCCCCGCCTCTCTGCACTCGCCCCGCCCCTCTCTGCCCCGCCCCTCTGCAATCGCCCCGCCCCGCCCCAGTTGC (deletion of Exon 2 to 8) TGTTGAGAACAGGCGCATAGCATTTGACATTTCAGTGCTACCGGAGCAAGCAATACTCCAGGTTTGTAGGAGTTTGTTGAATAGTTTTCTTACAATTTGAGTATAGTTTGGAGTTAGGGTAAGTATGTTTGCTTATAATTCCTTATTTTAAAAATTATCGGCTGGGCCGAAGTCTGGCTGATGAAGGGCGGAAGTAAGAGTACCAACTCCGCAATCATGGCTCAA |
Colony maintenance | |
References | PLoS Genet., 16(1):e1008585 (2020). 31961863 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Fbxl13F-box and leucine-rich repeat protein 135Fbxl13<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University |
Ordering Information | |
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Donor DNA | human hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Mouse a part of Fbxl13 gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 16(1):e1008585 (2020).The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |