Strain Data Sheet

RBRC10339

Strain Information

Image
BRC No.RBRC10339
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameSTOCK Fbxl13<em1Osb>
Former Common nameFbxl13-30983/wt
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeveloped by Keisuke Shimada and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2017. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed to BDF1. Mixed genetic background.
Strain descriptionSTOCK Fbxl13<em1Osb>; Deletin of Exon 2 to 8 of the Fbxl13CTCCCGTCCAGCCCTCTCATTTTACCCGGCCCCGGCCTGTACTGTCCTGCCCCGCCCCTCCCCGCTCTGCCCCGCCTCTCTGCACTCGCCCCGCCCCTCTCTGCCCCGCCCCTCTGCAATCGCCCCGCCCCGCCCCAGTTGC (deletion of Exon 2 to 8) TGTTGAGAACAGGCGCATAGCATTTGACATTTCAGTGCTACCGGAGCAAGCAATACTCCAGGTTTGTAGGAGTTTGTTGAATAGTTTTCTTACAATTTGAGTATAGTTTGGAGTTAGGGTAAGTATGTTTGCTTATAATTCCTTATTTTAAAAATTATCGGCTGGGCCGAAGTCTGGCTGATGAAGGGCGGAAGTAAGAGTACCAACTCCGCAATCATGGCTCAA
Colony maintenance
References
Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice.
Morohoshi A, Miyata H, Shimada K, Nozawa K, Matsumura T, Yanase R, Shiba K, Inaba K, Ikawa M
PLoS Genet., 16(1):e1008585 (2020). 31961863

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fbxl13
MGI:2443416		F-box and leucine-rich repeat protein 135Fbxl13<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Streptomyces alboniger puromycin resistant gene, Mouse a part of Fbxl13 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 16(1):e1008585 (2020).The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP, Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data