Strain Data Sheet

RBRC10338

Strain Information

Image
BRC No.RBRC10338
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameSTOCK Armc12<em2(Armc12/FLAG)Osb>
Former Common nameArmc12 C-FLAG
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeveloped by Keisuke Shimada and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2017. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed to BDF1. Mixed genetic background.
Strain descriptionThis strain has a C-terminal Flag tag at the Armc12 gene which was generated by the genome editing technique. Armc12<em2(Armc12/FLAG)Osb>; Flag tag insertion at C-terminus of the Armc12. AGATCCAGGCCTGCAAAGTCATAGTGAGCTTGCAGTGTCCCCAGGACCTGGGATCCCGACCCTCTTCCTGCCGACCTAGCCATTCCTGCTTTAAAACCGGGAAG(GGCGGCAGCGACTACAAAGACGATGACGACAAG)TGAAGTTAAGGAGATCCAATAAATGCGTACGGGAGGGAAGCTCGAGGCCTCCGGAAGCAAGCGTCTGTCCGTTGCCTCCCAGGACCTGGGTGGAGGTGCTGTTCATCA
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 118(6):e2018355118 (2021). 33542098

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Armc12armadillo repeat containing 1217Armc12<em2(Armc12/FLAG)Osb>endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University

Ordering Information

Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Escherichia coli Lac Z, T7 phage T7 promoter, Escherichia coli lac promoter, Mouse a part of Armc12 gene, synthetic FLAG tag
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data