Strain Data Sheet

RBRC10338

Strain Information

Image
BRC No.RBRC10338
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameSTOCK Armc12<em2(Armc12/FLAG)Osb>
Former Common nameArmc12 C-FLAG
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeveloped by Keisuke Shimada and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2017. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed to BDF1. Mixed genetic background.
Strain descriptionThis strain has a C-terminal Flag tag at the Armc12 gene which was generated by the genome editing technique. Armc12<em2(Armc12/FLAG)Osb>; Flag tag insertion at C-terminus of the Armc12. AGATCCAGGCCTGCAAAGTCATAGTGAGCTTGCAGTGTCCCCAGGACCTGGGATCCCGACCCTCTTCCTGCCGACCTAGCCATTCCTGCTTTAAAACCGGGAAG(GGCGGCAGCGACTACAAAGACGATGACGACAAG)TGAAGTTAAGGAGATCCAATAAATGCGTACGGGAGGGAAGCTCGAGGCCTCCGGAAGCAAGCGTCTGTCCGTTGCCTCCCAGGACCTGGGTGGAGGTGCTGTTCATCA
Colony maintenance
References
Ultrasound activates mechanosensitive TRAAK K(+) channels through the lipid membrane.
Sorum B, Rietmeijer R A, Gopakumar K, Adesnik H, Brohawn S G
Proc. Natl. Acad. Sci. USA, 118(6):e2018355118 (2021). 33542098

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Armc12
MGI:1914895		armadillo repeat containing 1217Armc12<em2(Armc12/FLAG)Osb> endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Streptomyces alboniger puromycin resistant gene, Escherichia coli Lac Z, T7 phage T7 promoter, Escherichia coli lac promoter, Mouse a part of Armc12 gene, synthetic FLAG tag
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP, Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data