Strain Data Sheet

RBRC10335

Strain Information

Image
BRC No.RBRC10335
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Oosp3<em1Osb>
Former Common nameOosp3-26/-26
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Abbasi Ferheen, Masashi Mori, Haruhiko Miyata and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2016. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionOosp3<em1Osb>; 26 bp deletion at Exon 2aacttctgagtgttttgcaagctgattctatttgaggaatatcaatagaaagaaaagaggagagtcatatcatgagagtaatcatctttgcccatatccctaaactctattaattttggccaaattgggtttttcatttctcctaatttggatcaaagttctttgctatgtattaaggtggggcagctgattgttttttcgtctctttcaacagTATCCATGGAATGTGATTACTTTACATTCCGTGTTATAGCTAAAAGGGCACTGTTTTATCCAGATGATCTCATTGGCCCTGATGAATTATTATTAGGGGCAAGCTGCCCAGTAACTTCCATAAGGCCAGATGAACTTGAGTTCTATTATGATATCCATTCCTGTGGGACTTTCATACAGgtaagagaaggaactgtatttcttggtatcatctgtaaagtcttctcaagtgttgttttctgtccttgtcttaatatatacaaaacactggtattaccatcctagtgccttagtctaagagaaacacaaagaattttttgttacttctattcattcaccagagacttgccatgtataggataat
Colony maintenance
References
CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice.
Abbasi F, Kodani M, Emori C, Kiyozumi D, Mori M, Fujihara Y, Ikawa M
Cells, 9(4):821 (2020). 32231122

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Oosp3
MGI:2684943		oocyte secreted protein 319Oosp3<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Oosp3 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cells. 2020 Mar 28;9(4). pii: E821.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP, Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data