Strain Data Sheet

RBRC10332

Strain Information

Image
BRC No.RBRC10332
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Dcst1<em2Osb>
Former Common nameDcst1-1/wt
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2015. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. Homozygous males are infertile.
Strain descriptionDcst1<em2Osb>; 1 bp deletion at Exon 1 cagcgttgactatggctgctgggacccaaggccagtgtgtctccaggaaaggcctggagagtcttcaggttctatggacctggagttcttcattctgttctaaggttctgaaggcttgtaccatgggcgtgtctcAGAACCCACTACCCAGTTGTTCTGAGGGCTCCCGGAGCTGAGGAGTTGAAGGACACAGGCCAGTCGTGGGCTGTATGTGAAGCAAAAGACAGGGGCGGCATGGCGTTCCTCTCATCAACACTTCACAGCCTAGGGATCTTTGAAAAGATTTCCAGGATAAAAGAGGTTCTCAAGAATAGGCTGCTGGACCTTACAAAGCGTCGGGACCAAGCACGGGAGCAACAAAGGAAACGGCCCCACACCAgtgagcagccctcagatgccagcctcgttccagcatcccccaggctcctctttcctctctcctctgctatggggccatccacctcccatcctctctcagttcagccctcttctctcctcctccactctggccttactcctcggcatccctctttctagcgtgcttcacgcccccaccaccaggattccccagtgtctgagcctctcagaatctcagcgcctggggctggagagatggc
Colony maintenance
ReferencesCommun Biol., 5(1):332 (2022). doi: 10.1038/s42003-022-03289-w. 35393517

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dcst1DC-STAMP domain containing 13Dcst1<em2Osb>endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University

Ordering Information

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse　a part of Dcst1 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Commun Biol Apr 7;5(1):332.(2022) doi: 10.1038/s42003-022-03289-w.The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data