Strain Information | |
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Image | |
BRC No. | RBRC10302 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C3;B6-Lrit1<tm1c(EUCOMM)Hmgu>/Tfur |
Former Common name | Lrit1-flox |
H-2 Haplotype | |
ES Cell line | JM8.N4 [C57BL/6N] |
Background strain | |
Appearance | |
Strain development | Developed by Takahisa Furukawa, Institute for Protein Research, Osaka University in 2017. JM8.N4 ES cells derived from C57BL6/N were used. C3H and B6 mixed genetic background. Mutations of Rd1(Pde6b) and Rd8(Crb1) were replaced with their normal alleles. |
Strain description | Lrit1(leucine-rich repeat, immunoglobulin-like and transmembrane domains 1)flox mice.A pair of loxP sites flank Exon 2. IRES-LacZ/Neo cassette was removed by crossing with B6-Tg(CAG-FLPe)37. |
Colony maintenance | |
References | Cell Reports, 22, 3548-3561 (2018). 29590622 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Lrit1leucine-rich repeat, immunoglobulin-like and transmembrane domains 114Lrit1<tm1c(EUCOMM)Hmgu>targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site14Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP |
Ordering Information | |
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Donor DNA | yeast FRT (flipase recombination target) site, Bacteriophage P1 loxP sites, Mouse Lrit1 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Reports, 22, 3548-3561 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takahisa Furukawa) and enter into a Material Transfer Agreement with Osaka University. The RECIPIENT shall acknowledge the EUCOMM as the Provider of the ES cells to generate KO mice. |
Depositor | Takahisa Furukawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |