Strain Information | |
---|---|
Image | |
BRC No. | RBRC10295 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.B6CB-Ahcyl1<tm1.1Kmik>/2 |
Former Common name | IRBIT KO |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2009. TT2 ES cells were used and this line was backcrossed to C57BL/6 for 15 generations. C57BL/6 congenic line. |
Strain description | Ahcyl1 (S-adenosylhomocysteine hydrolase-like 1) floxed mice. FRT-flanked Neo gene is placed at intron 1 of the Ahcyl1 gene. A pair of loxP sites flank the FRT-flanked Neo and exon 2 of the Ahcyl1 gene. The neo and exon 2 were removed by crossing with a Cre line. TT2 ES cells were used and then this line was backcrossed to C57BL/6 for 15 generations. C57BL/6 congenic line. Homozygous mutants tend to be small in their body size. Two sublines are available as RBRC10294 and RBRC10295. This is subline #2. |
Colony maintenance | |
References | Proc. Natl. Acad. Sci. USA, 103(25) 9542-9547 (2006). 16769890 Mol Cell. 22(6) 795-806 (2006). 16793548 Proc. Natl. Acad. Sci. USA, 112(17):5515-5520 (2015). 25922519 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ahcyl1S-adenosylhomocysteine hydrolase-like 13Ahcyl1<tm1.1Kmik>targeted mutation 1.1, Katsuhiko Mikoshiba Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP |
Ordering Information | |
---|---|
Donor DNA | P1 phage loxP sequence, Mouse Ahcyl1(deleted) |
Research application | Cre/loxP system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Katsuhiko Mikoshiba (RIKEN) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
---|
No Data |