Strain Information | |
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Image | |
BRC No. | RBRC10280 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.B6CB-Ahcyl1<tm1Kmik>/1 |
Former Common name | IRBIT flox mice |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2009. TT2 ES cells were used and this subline #1 was backcrossed to C57BL/6 for 13 generations. C57BL/6 congenic line. |
Strain description | hcyl1 (S-adenosylhomocysteine hydrolase-like 1) floxed mice. FRT-flanked Neo gene is placed at intron 1 of the Ahcyl1 gene. A pair of loxP sites flank the FRT-flanked Neo and exon 2 of the Ahcyl1 gene. TT2 ES cells were used and this subline #1 was backcrossed to C57BL/6 for 13 generations. C57BL/6 congenic line. |
Colony maintenance | |
References | Mol Cell. 22(6):795-806 (2006). 16793548 Proc. Natl. Acad. Sci. USA, 103(25):9542-9547 (2006). 16769890 Proc. Natl. Acad. Sci. USA, 112(17):5515-5520 (2015). 25922519 |
Health Report | |
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Examination Date / Room / Rack | 2022/07/11Room:3-BRack:G |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ahcyl1S-adenosylhomocysteine hydrolase-like 13Ahcyl1<tm1Kmik>targeted mutation 1, Katsuhiko Mikoshiba Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene3mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | P1 phage loxP sites, Saccharomyces cerevisiae frt sites, mouse PGK promoter, Escherichia coli neomycin-resistant gene (Pro/Neo/3'end), mouse PGK pA, mouse Ahcyl1 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Katsuhiko Mikoshiba (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |