Strain Information | |
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Image | |
BRC No. | RBRC10275 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6D2-Dcst1<em1Nino> |
Former Common name | Dcst1-KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Naokazu Inoue, Fukushima Medical University in 2017. B6D2 genetic background. |
Strain description | Dcst1 (DC-STAMP domain containing 1) mutant mice. 5 bp deletion at Exon 5. Homozygous mutant males are infertile.Dcst1<em1Nino>: 5 bp deletion at Exon 5.AGATCAACAACACCCGATCCgcctggcgagtctccacagcccctctgcgggcagtgttcaagggcatggtggtcagcatcctgtatagtccctatccccaggactgagttgggttggactgggaacatggccctggatatatggtcctcgcagggaactcctattatattttgtatcactatccccagacattgcagagcctttcctgatcttggggtgttgaaaaaccaggaggtcaacataccatagggacagacatacttgcatatttccagatacccacatgggcaggacttgctcagctgaatgagaatgttaggaaattgggaatattgacttttggagtccagaggatcagaaaatggaataTTTGGGGCTACTGGCTGATG |
Colony maintenance | |
References | Elife, 10:e66313 (2021). 33871360 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter |
Ordering Information | |
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Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin] |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Elife. 2021 Apr 19;10:e66313. |
Depositor | Naokazu Inoue (Fukushima Medical University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |