Strain Data Sheet

RBRC10275

Strain Information

Image
BRC No.RBRC10275
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Dcst1<em1Nino>
Former Common nameDcst1-KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Naokazu Inoue, Fukushima Medical University in 2017. B6D2 genetic background.
Strain descriptionDcst1 (DC-STAMP domain containing 1) mutant mice. 5 bp deletion at Exon 5. Homozygous mutant males are infertile.Dcst1<em1Nino>: 5 bp deletion at Exon 5.AGATCAACAACACCCGATCCgcctggcgagtctccacagcccctctgcgggcagtgttcaagggcatggtggtcagcatcctgtatagtccctatccccaggactgagttgggttggactgggaacatggccctggatatatggtcctcgcagggaactcctattatattttgtatcactatccccagacattgcagagcctttcctgatcttggggtgttgaaaaaccaggaggtcaacataccatagggacagacatacttgcatatttccagatacccacatgggcaggacttgctcagctgaatgagaatgttaggaaattgggaatattgacttttggagtccagaggatcagaaaatggaataTTTGGGGCTACTGGCTGATG
Colony maintenance
ReferencesElife, 10:e66313 (2021). 33871360

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dcst1DC-STAMP domain containing 13Dcst1<em1Nino>endonuclease-mediated mutation 1, Naokazu Inoue

Ordering Information

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Elife. 2021 Apr 19;10:e66313.
DepositorNaokazu Inoue (Fukushima Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data