Strain Information | |
---|---|
Image | |
BRC No. | RBRC10274 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6D2-Cd9<em1Nino> |
Former Common name | Cd9-KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Naokazu Inoue, Fukushima Medical University in 2017. B6D2 genetic background. |
Strain description | Cd9 (CD9 antigen) mutant mice. 10 bp deletion at Exon 4. Homozygous mutant females are infertile.Cd9<em1Nino>: 10 bp deletion at Exon 4.CCCTCATGATGCTGGTTGGTttcctgggctgctgtggagctgtacaagagtcccagtgcatgctgggattggtaagtattccttctatagagtagctagccagccttaccttaatcctttcccaaaatgtcaccaggcatttgcacagaacaggtagaaccggtggagtcaggaaggctcctgggccactggctgtgttcagcctctcaccatccttacttcaggcccagtcccgtctgcctggctcccaccaccatcttgccatggccggccctgtattcctaatcacttcctttatgcacctaaatgccaggactgtatttccgcctttctcaaaggccatgtttgctttctcccgagtccctaggtccttttgccttctctccttattttaaaaattctttccgtcttgcccagacgccacggagtgaatgcgctgcctttgtttccccgatctcggtggtatttgtgtgtgggcagtggtggtttattgctgcccgactctaaactaacatccccaagtaccagtgtaggggcagcagatcacattttccccccagaattcatggcttctctgctttttgcttaacctcttcctgcctgtccatctgtgccacccgcacgctaactgttctgcaagcctttggtattgctgactggcttgctggagtctctctcaccacgtcctttgtgtcttctagttcttcgggttcctcttggtgatattcgccattgagatagccgccgcCGTCTGGGGCTATACCCACA |
Colony maintenance | |
References | Development. 2020 Jul 14;dev.189985. 32665248 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter |
Ordering Information | |
---|---|
Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin] |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. |
Depositor | Naokazu Inoue (Fukushima Medical University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
---|
No Data |