Strain Data Sheet
RBRC10267
Strain Information | |
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| Image | |
| BRC No. | RBRC10267 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129-Mlc1<tm1kftnk> |
| Former Common name | Mlc1 STOPtetO knockin |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | 129S6/B6 hybrid ESを使用。コロンビア大学で田中謙二が作成し、生理研へ移動させた。生後1年で白質に空胞が生じる。 |
| Strain description | アストロサイト特異的に発現する遺伝子、Mlc1の翻訳開始部位の直前にNeoSTOPtetOがノックインしてある。Homozygoteはノックアウトマウスになる。 |
| Colony maintenance | |
| References | Glia, 65(1), 150-168 (2017). doi: 10.1002/glia.23084. Epub 2016 Oct 17. 27748972 |
Health Report | |
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| Examination Date / Room / Rack | 2024/01/09Room:3-BRack:J 2023/10/10Room:3-BRack:J 2023/07/10Room:3-BRack:J 2023/04/10Room:3-BRack:J 2023/01/10Room:3-BRack:J 2022/10/11Room:3-BRack:J 2022/07/11Room:3-BRack:J |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Mlc1megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human)11Mlc1<tm1kftnk>targeted mutation 1, Kenji Tanaka Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter His3His3 (S. cerevisiae)11His3 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tetOtetracycline operator (E. coli)11tetOCMV promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA11 |
Ordering Information | |
|---|---|
| 供与核酸 | phage P1 loxP sites, yeast FRT (flippase recombination target) sites, mouse PGK promoter, synthetic bacterial EM7 promoter (T7 phage), E. coli Neomycin resistance gene, yeast His3 3' UTR, SV40 polyA signal, E. coli Tetracycline operator site, CMV minimal promoter, mouse Mlc1 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Tet system |
| 提供条件 | 条件を付加しない。 |
| Depositor | 池中 一裕（大学共同利用機関法人自然科学研究機構） |
| Strain Status |  生体 凍結胚 凍結精子 |
| Strain Availability | 凍結胚を1ヶ月以内に提供可能 生体マウスを3~6ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |