Strain Information | |
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Image | |
BRC No. | RBRC10202 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | CB17.Cg-Sema4D<tm1In> |
Former Common name | CB17-Sema4D-KO |
H-2 Haplotype | |
ES Cell line | EB3 [129/Ola] |
Background strain | |
Appearance | |
Strain development | Developed by Shinobu Inagaki, Osaka University Graduate School of Medicine, Division of Health Sciences. EB3 ES cells derived from 129P2/OlaHsd were used. This strain was backcrossed to C.B-17/lcr-+/+Jcl for 10 generations. |
Strain description | Sema4d [sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D] knockout mice. IRES-tauLacZ-Neo-pA fragment was inserted between exon 1 and exon 2. C.B-17/lcr congenic strain. |
Colony maintenance | |
References | Glia, 6, 2249-2259 (2015). 26202989 J. Neurosci. Res., 87, 2833-2841 (2009). 19472224 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Sema4dsema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D13Sema4d<tm1In>targeted mutation 1, Shinobu Inagaki Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)13 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)13 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene13mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter taubovine tau protein cDNA13tau Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal13 |
Ordering Information | |
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Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), bovine tau cDNA, E. coli LacZ, SV40 polyA signal, mouse phosphoglycerate kinase promoter (Pgk promoter), E. coli Neomycine resistant gene, mouse Sema4D genomic DNA |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci. Res., 87, 2833-2841 (2009). Glia, 6, 2249-2259 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
Depositor | Drs. Tatsuo Furuyama and Shinobu Inagaki (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |