Strain Data Sheet

RBRC10197

Strain Information

Image
BRC No.RBRC10197
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Trp53<em1.1Rbrc>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentRIKEN BRC developed this strain by crossing a floxed Trp53 strain (RBRC09921) with a Cre strain. This was funded through the FY2016 AMED-NBRP fundamental technology upgrading program.
Strain descriptionTrp53 knockout mice in the C57BL/6N genetic background. A genomic region from Exon 2 to Exon 9 of the Trp53 was deleted.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2023/07/24Room:3-DRack:L
2023/04/24Room:3-DRack:L
2023/01/23Room:3-DRack:L
2022/10/25Room:3-DRack:L
2022/07/25Room:3-DRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Trp53transformation-related protein 5311Trp53<em1.1Rbrc>endonuclease mediated mutation 1.1, RIKEN BRC

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Ordering Information

供与核酸Phage P1 loxP site, mouse Trp53 genomic DNA
Research applicationCre/loxP system
提供条件条件を付加する。
1, 研究成果の公表にあたってRIKEN BRCへの謝辞の表明を必要とする。
2, 営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
3, 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
DepositorRIKEN BRC（国立研究開発法人理化学研究所）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data