Strain Data Sheet

RBRC10181

Strain Information

Image
BRC No.RBRC10181
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6.129P2-Gt(ROSA)26Sor<em1(CAG-EGFP<*1>)Maoh>
Former Common namedelta eGFP Tg #197
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hiromi Miura and Masato Ohtsuka, Tokai University School of Medicine in 2016. This strain was generated by the Genome editing using RBRC05466, B6.129P2-Gt(ROSA)26Sor<tm2.1(CAG-EGFP)Maoh>.
Strain descriptionA strain harboring one base pair deletion in the CAG-EGFP of the RBRC05466, B6.129P2-Gt(ROSA)26Sor<tm2.1(CAG-EGFP)Maoh>. No fluorescence can be detected by this mutation, but fluorescence can be recovered by its correction by genome editing. C57BL/6J congenic line.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<em1(CAG-EGFP<*1>)Maoh>endonuclease-mediated mutation 1, Masato Ohtsuka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox2272phage P1 loxP variant6lox2272

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal6

Ordering Information

Donor DNAyeast FRT (flipase recombination target) site, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Aequorea victoriae GFP cDNA, Simian virus 40 (SV40) polyA additional signal, Phage P1 lox2272 site, mouse Gt(ROSA)26Sor genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasato　Ohtsuka (Tokai University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data