Strain Data Sheet
RBRC10181
Strain Information | |
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| Image | |
| BRC No. | RBRC10181 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | B6.129P2-Gt(ROSA)26Sor<em1(CAG-EGFP<*1>)Maoh> |
| Former Common name | delta eGFP Tg #197 |
| H-2 Haplotype | |
| ES Cell line | E14TG2a [129P2/OlaHsd] |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | Developed by Hiromi Miura and Masato Ohtsuka, Tokai University School of Medicine in 2016. This strain was generated by the Genome editing using RBRC05466, B6.129P2-Gt(ROSA)26Sor<tm2.1(CAG-EGFP)Maoh>. |
| Strain description | A strain harboring one base pair deletion in the CAG-EGFP of the RBRC05466, B6.129P2-Gt(ROSA)26Sor<tm2.1(CAG-EGFP)Maoh>. No fluorescence can be detected by this mutation, but fluorescence can be recovered by its correction by genome editing. C57BL/6J congenic line. |
| Colony maintenance | |
| References | |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 6 | EGFP | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<em1(CAG-EGFP<*1>)Maoh> | endonuclease-mediated mutation 1, Masato Ohtsuka | |||
| lox2272 | phage P1 loxP variant | 6 | lox2272 | ||||
| SV40 polyA signal | 6 | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | yeast FRT (flipase recombination target) site, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Aequorea victoriae GFP cDNA, Simian virus 40 (SV40) polyA additional signal, Phage P1 lox2272 site, mouse Gt(ROSA)26Sor genomic DNA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Masato Ohtsuka (Tokai University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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