Strain Data Sheet
RBRC10171
Strain Information | |
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| Image | |
| BRC No. | RBRC10171 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6;Cg-Gt(ROSA)26Sor<tm3(rtTA3)Yasu> Col1a1<tm4(tetO-OSKM,-mCherry)Yasu> |
| Former Common name | Rosa26-LSL-rtTA3/ Col-tetO-OSKM |
| H-2 Haplotype | |
| ES Cell line | V6.5 [(C57BL/6J x 129S4/SvJae)F1] |
| Background strain | |
| Appearance | |
| Strain development | インジェクションの受精卵系統：C57BL/6ジーンターゲティングに用いたES細胞名：V6.5開発者：柴田博史開発年：2015ヘテロとWTの交配により系統を維持 |
| Strain description | 組み換え酵素Creが発現した細胞でrtTA3の発現誘導が可能さらにDoxycycline 投与により、rtTA3が発現した細胞でOSKM, mCherry の発現誘導が可能。 |
| Colony maintenance | |
| References | Nature Commun., 9, 2081 (2018). 29802314 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Col1a1collagen, type I, alpha 111Col1a1<tm4(tetO-OSKM,-mCherry)Yasu>targeted mutation 4, Yasuhiro Yamada Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm3(rtTA3)Yasu>targeted mutation 3, Yasuhiro Yamada Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter E2AEquine rhinitis A virus (ERAV) 2A peptideE2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2Rabbit globin polyAHBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hyghygromycin phasphotransferase (E. coli)Hyg Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter P2Aporcine teschovirus-1 2A peptideP2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter T2AThosea asigna virus 2A peptide (insect)T2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mCherryRed Fluorescent Protein (Discosoma sp.)mCherry Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (Klebsiella pneumoniae)mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA |
Ordering Information | |
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| 供与核酸 | adenovirus type2 Splicing acceptor (SA) [Rosa26, Col1a1 locus], P1 Bacteriophage loxP sites, mouse Pgk1 promoter, Klebsiella pneumoniae (Bacterial transposon Tn5) Neomycin registance gene (Neo), SV40 pA, Escherichia coli K-12 strain derivative rtTA3, Escherichia coli K-12 strain derivative Hygromycin registance gene, CMV promoter, E. coli tetO (tetracycline operator), yeast Frt site, Escherichia coli ampicillin registance gene, mouse Oct3/4, mouse Sox2, mouse Klf4, mouse c-Myc, Porcineteschovirus-1 P2A,Thoseaasignavirus T2A,Equine rhinitis A virus E2A, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Discosoma sp. mCherry cDNA, Rabbit globin polyA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System Tet system |
| 提供条件 | 条件を付加する。 研究成果の公表にあたって寄託者の指定する文献を引用する。Nature Commun., 9, 2081 (2018). 学術機関の学術研究に限る。 |
| Depositor | 山田 泰広（国立大学法人京都大学） |
| Strain Status |  凍結胚 凍結精子 |
| Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |