Strain Data Sheet

RBRC10170

Strain Information

Image
BRC No.RBRC10170
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Pdx1<tm1(cre)Yasu>
Former Common namePdx1 ires-Cre
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentインジェクションの受精卵系統：C57BL/6ジーンターゲティングに用いたES細胞名：V6.5開発者：柴田　博史開発年：2015ホモ同士の交配により系統を維持
Strain descriptionPdx1 陽性細胞でCre タンパクが発現する。
Colony maintenance
ReferencesNature Commun., 9, 2081 (2018). 29802314

Health Report

Examination Date / Room / Rack2024/03/11Room:3-2Rack:A
2024/01/22Room:3-DRack:A
2023/12/11Room:3-2Rack:A
2023/10/23Room:3-DRack:A
2023/09/11Room:3-2Rack:A
2023/07/24Room:3-DRack:A
2023/06/26Room:3-2Rack:A
2023/06/13Room:3-2Rack:A
2023/04/24Room:3-DRack:A
2023/03/13Room:3-2Rack:A
2023/01/23Room:3-DRack:A
2022/12/12Room:3-2Rack:A
2022/10/25Room:3-DRack:A
2022/09/12Room:3-2Rack:A
2022/07/25Room:3-DRack:A
2022/06/13Room:3-2Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pdx1pancreatic and duodenal homeobox 15Pdx1<tm1(cre)Yasu>targeted mutation 1, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA5HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase5cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)5puromouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxBacteriophage D6 rox site5rox

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxBacteriophage D6 rox site5rox

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal5

Ordering Information

供与核酸Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), P1 Bacteriophage Cre, Rabbit globin pA, D6 Bacteriophage Rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Streptomyces alboniger puromycin resistance gene, Simian virus (SV40) pA, mouse Pdx1 genomic DNA
Research applicationCre/loxP system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Nature Commun., 9, 2081 (2018).
学術機関の学術研究に限る。
Depositor山田　泰広（国立大学法人京都大学）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data