Strain Data Sheet

RBRC10144

Strain Information

Image
BRC No.RBRC10144
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Tppp3<tm2(cre/ERT2)Yasu>
Former Common name
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentインジェクションの受精卵系統：B6D2F1ジーンターゲティングに用いたES細胞名：V6.5開発者：河村真吾開発年：2013ホモ同士の交配により系統を維持
Strain descriptionTppp3が発現する細胞で組み換え酵素CreERT2が発現する。タモキシフェン投与により、Cre-LoxP組み換え反応が誘導できる。
Colony maintenance
ReferencesNat. Commun., 10(1):3999 (2019). 31488818
Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. （in revision）

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tppp3tubulin polymerization-promoting protein family member 38Tppp3<tm2(cre/ERT2)Yasu>targeted mutation 2, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BsdAspergillus terreus blasticidin resistance gene (Bsd)8Bsdmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)8ERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA8HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)8

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase8cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxBacteriophage D6 rox site8rox

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxBacteriophage D6 rox site8rox

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal8

Ordering Information

供与核酸Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), P1 Bacteriophage Cre, human Estrogen receptor binding domain (ERT2)cDNA, Rabbit globin pA, D6 Bacteriophage rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus Blasticidin S deaminase gene (Bsd), Simian virus (SV40) pA, mouse Tppp3 genomic DNA
Research applicationCre/loxP system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Nat Commun. 2019 Sep 5;10(1):3999.
学術機関の学術研究に限る。
Depositor山田　泰広（国立大学法人京都大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data