Strain Information | |
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Image | |
BRC No. | RBRC10143 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6;Cg-Sox10<tm1(cre/ERT2)Yasu> |
Former Common name | Sox10-CreERT2 |
H-2 Haplotype | |
ES Cell line | V6.5 [(C57BL/6J x 129S4/SvJae)F1] |
Background strain | |
Appearance | |
Strain development | インジェクションの受精卵系統:B6D2F1ジーンターゲティングに用いたES細胞名:V6.5開発者:河村真吾開発年:2013ホモ同士の交配により系統を維持。 |
Strain description | Sox10が発現する細胞で組み換え酵素CreERT2が発現する。タモキシフェン投与により、Cre-LoxP組み換え反応が誘導できる。 |
Colony maintenance | |
References | Nat. Commun., 10(1):3999 (2019). 31488818 Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. (in revision) |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Sox10SRY (sex determining region Y)-box 1015Sox10<tm1(cre/ERT2)Yasu>targeted mutation 1, Yasuhiro Yamada Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter BsdAspergillus terreus blasticidin resistance gene (Bsd)15Bsdmouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)15ERT2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyA15HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)15 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase15cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site15rox Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site15rox Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal15 |
Ordering Information | |
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供与核酸 | Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), P1 Bacteriophage Cre, human Estrogen receptor binding domain (ERT2)cDNA, Rabbit globin pA, D6 Bacteriophage rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus Blasticidin S deaminase gene (Bsd), Simian virus (SV40) pA, mouse Sox10 genomic DNA |
Research application | Cre/loxP system |
提供条件 | 条件を付加する。 研究成果の公表にあたって寄託者の指定する文献を引用する。Nat Commun. 2019 Sep 5;10(1):3999. 学術機関の学術研究に限る。 |
Depositor | 山田 泰広(国立大学法人京都大学) |
Strain Status | 凍結胚 凍結精子 |
Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |