Strain Data Sheet
RBRC10141
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10141 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6;Cg-Gt(ROSA)26Sor<tm2(rtTA3,-Venus)Yasu> Col1a1<tm2(tetO-EWSR1/ATF1,-mCherry)Yasu> |
| Former Common name | Rosa26-loxP-Stop-loxP-rtTA/Col-tetO-EWS/ATF1 |
| H-2 Haplotype | |
| ES Cell line | KH2[F1:C57BL/6 x 129Sv] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Shingo Komura and his colleagues in 2013, and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. KH2 ES cells derived from ((C57BL/6(f)× 129/SV(m))F1 were used. Mixed genetic background. |
| Strain description | Compound mouse line harboring the following two loci; 1, loxP-Pgk-neo-loxP-rtTA3-ires-Venus is knocked in at the Rosa26 locus, 2, tetO-EWSR1/ATF1-ires-mCherry is knocked in at the Col1a1 locus. |
| Colony maintenance | |
| References | Nat. Commun., 10(1):3999 (2019). 31488818 Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. （in revision） |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |
|---|---|
| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2(rtTA3,-Venus)Yasu>targeted mutation 2, Yasuhiro Yamada Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)6Venus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter hyghygromycin phasphotransferase (E. coli)6hyg Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter rtTA3E. coli Reverse tetracycline transactivator (Mutated tetrepressor DNA-binding domain and the transactivation domain of VP-16)6rtTA3 |
Ordering Information | |
|---|---|
| Donor DNA | adenovirus type2 Splicing acceptor (SA), P1 Bacteriophage loxP sites, mouse PGK promoter, Klebsiella pneumoniae (Bacterial transposon Tn5) Neomycin resistance gene(Neo), Simian virus (SV40) polyA, Escherichia coli K-12 rtTA3, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Aequorea victoria Venus cDNA, Bovine growth hormone (bGH) polyA,mouse Rosa26 genomic DNA, E. coli tetO (tetracycline operator), human EWS cDNA, human ATF1 cDNA, Influenza virus A HA tag, Discosoma sp. mCherry cDNA, Rabbit globin polyA, Escherichia coli K-12 hygromycin resistance gene, CMV promoter, yeast FRT (flipase recombination target) site, Escherichia coli ampicillin resistant gene, mouse Col1A1 genomic DNA |
| Research application | FLP/frt system Fluorescent Proteins/lacZ System Tet system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Commun. 2019 Sep 5;10(1):3999.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
| Depositor | Yasuhiro Yamada (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
|
BRC mice in Publications |
|---|
| No Data |