Strain Data Sheet

RBRC10131

Strain Information

Image
BRC No.RBRC10131
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Tesmin<em1Osb> Tg(CAG-Tesmin)1Osb
Former Common nameMtl5<-316/wt>, CAG-Mtl5#3, B6D2-Mtl5<em1Osb> Tg(CAG-Mtl5)1Osb
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Asami Oji, Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2016. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionCompound mutant harboring Mtl5 KO mutation and transgene expressing Mtl5 under the control of the CAG promoter.
Colony maintenance
ReferencesBiol. Reprod., 102(4):975-983 (2020). doi: 10.1093/biolre/ioaa002. 31916570

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tesmintestis expressed metallothionein like19Tesmin<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka UniversityMtl5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tesmintestis expressed metallothionein likeUNTesminMtl5CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Ordering Information

Donor DNAmouse Tesmin cDNA, chicken beta-actin promoter, rabbit beta-globin polyadenylation signal, CMV IE enhancer, human hU6 promoter, SV40 nuclear localization signal, Streptococcus pyogenesSpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 102(4):975-983 (2020). doi: 10.1093/biolre/ioaa002.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data