Strain Data Sheet

RBRC10067

Strain Information

Image
BRC No.RBRC10067
TypeTransgene and Spontaneous MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-c/c Tg(CAG-iRFP)#846Utr
Former Common nameiRFP Tg #846
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yoshihiro Miwa, University of Tsukuba in 2012. A DNA construct was injected into fertilized eggs derived from B6D2F1. Mice were backcrossed to B6(Cg)Tyr<c2-J> for 17 generations. C57BL/6J congenic line.
Strain descriptionTransgenic mice expressing iRFP under the control of the CAG promoter. This line (B6.Cg-c/c Tg(CAG-iRFP)#846Utr) exhibits stronger iRFP expression in heart than RBRC10068 (B6.Cg-c/c Tg(CAG-iRFP)#867Utr).
Colony maintenance
References
In vivo image analysis using iRFP transgenic mice.
Tran M T, Tanaka J, Hamada M, Sugiyama Y, Sakaguchi S, Nakamura M, Takahashi S, Miwa Y
Exp. Anim., 63, 311-319 (2014). 25077761

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tyr
MGI:98880		tyrosinase7Tyr<c-2J>
MGI:1855985		albino 2 Jackson
  • oculocutaneous albinism type 1A(DisGeNET, MedGEN)

  • oculocutaneous albinism type 1B(MedGEN)

  • oculocutaneous albinism(DisGeNET)
    • iRFP phytochrome-based near-infrared fluorescent protein (iRFP)UNiRFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
    SV40 polyAUN

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal aqueous drainage system morphology (MP:0005198)

  • abnormal eye physiology (MP:0005253)

  • abnormal intraocular pressure (MP:0005257)

  • abnormal keratinocyte apoptosis (MP:0001224)

  • abnormal melanosome morphology (MP:0005075)
    • more 14 phenotypes
  • abnormal retina ganglion layer morphology (MP:0005241)

  • abnormal retina morphology (MP:0001325)

  • abnormal retina photoreceptor morphology (MP:0001004)

  • abnormal vision (MP:0002090)

  • absent coat pigmentation (MP:0005171)

  • absent hair follicle melanin granules (MP:0000394)

  • absent skin pigmentation (MP:0001189)

  • decreased a-wave amplitude (MP:0012143)

  • decreased b-wave amplitude (MP:0012144)

  • decreased retina photoreceptor cell number (MP:0001327)

  • increased cellular sensitivity to ionizing radiation (MP:0004227)

  • normal phenotype (MP:0002873)

  • ocular albinism (MP:0006159)

  • retina degeneration (MP:0001326)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Rhodopseudomonas palustris iRFP DNA, SV40 Poly(A) site
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 63, 311-319 (2014).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR Dr. Yoshihiro Miwa (ymiwa@md.tsukuba.ac.jp) of University of Tsukuba using the Approval Form.
    DepositorYoshihiro Miwa (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Feb 2019

    BRC mice in Publications

    No Data