Strain Information | |
---|---|
Image | |
BRC No. | RBRC10067 |
Type | Transgene and Spontaneous Mutation |
Species | Mus musculus |
Strain name | B6.Cg-c/c Tg(CAG-iRFP)#846Utr |
Former Common name | iRFP Tg #846 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Yoshihiro Miwa, University of Tsukuba in 2012. A DNA construct was injected into fertilized eggs derived from B6D2F1. Mice were backcrossed to B6(Cg)Tyr<c2-J> for 17 generations. C57BL/6J congenic line. |
Strain description | Transgenic mice expressing iRFP under the control of the CAG promoter. This line (B6.Cg-c/c Tg(CAG-iRFP)#846Utr) exhibits stronger iRFP expression in heart than RBRC10068 (B6.Cg-c/c Tg(CAG-iRFP)#867Utr). |
Colony maintenance | |
References | In vivo image analysis using iRFP transgenic mice. Tran M T, Tanaka J, Hamada M, Sugiyama Y, Sakaguchi S, Nakamura M, Takahashi S, Miwa Y Exp. Anim., 63, 311-319 (2014). 25077761 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |||||||
---|---|---|---|---|---|---|---|
Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Tyr | tyrosinase | 7 | Tyr | albino 2 Jackson | |||
SV40 polyA | UN | ||||||
iRFP | phytochrome-based near-infrared fluorescent protein (iRFP) | UN | iRFP | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Phenotype | |
---|---|
Annotation by Mammalian phenotyhpe ontology | more 14 phenotypes |
Detailed phenotype data |
Ordering Information | |
---|---|
Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Rhodopseudomonas palustris iRFP DNA, SV40 Poly(A) site |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 63, 311-319 (2014).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR Dr. Yoshihiro Miwa (ymiwa@md.tsukuba.ac.jp) of University of Tsukuba using the Approval Form. |
Depositor | Yoshihiro Miwa (University of Tsukuba) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Feb 2019 |
BRC mice in Publications |
---|
No Data |