Strain Data Sheet

RBRC10065

Strain Information

Image
BRC No.RBRC10065
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6D2-Tg(CAG-Hist1h3f)17Ibbr
Former Common nameFlag-H3.2 transgenic mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Fugaku Aoki, Machika Kawamura and Tomohiko Akiyama, The University of Tokyo in 2013. Fertilized eggs derived from C57BL/6 (male) X B6D2F1 (female) were used. Backcrossed to C57BL/6J for 3 generations. Mixed genetic background.
Strain descriptionTransgenic mice expressing mouse histone H3.2(Hist1h3f histone cluster 1, H3f)under the control of the CAG promoter. Flag tag is fused to histone H3.2 N-terminally.
Colony maintenanceCarrier x Noncarrier [or Crossing to C57BL/6JJmsSlc]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAG FLAG tag (synthetic)UNFLAG
Hist1h3f histone cluster 1, H3fUNHist1h3f transgene insertion 17, Fugaku AokiCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Flag-tag cDNA, Mouse Histone H3.2 cDNA (Hist1h3f)
    Research application
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorFugaku Aoki (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data