Strain Data Sheet

RBRC10054

Strain Information

Image
BRC No.RBRC10054
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Aqp4<tm1Tsna>
Former Common nameAQP4 Neo, Aqp4 flox
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrl
Appearance
Strain developmentB6由来ES細胞RENKA株を用いて、flox系統(AQP4Neo)を作製。兄弟交配により系統維持。開発者　辻田実加。新潟大学脳研究所細胞神経生物学教室にて作成。特別な所見無し。
Strain descriptionAquaporin 4 Conditional Knockout のためのflox系統
Colony maintenance
ReferencesNeurosci. Res., 64, 208-212 (2009). 19428702

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Aqp4aquaporin 418Aqp4<tm1Tsna>targeted mutation 1, Tsutomu Nakada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)18mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal18

Ordering Information

供与核酸phage P1 loxP sites, Saccharomyces cerevisiae FRT sites, mouse Pgk1 promoter, E. coli aminoglycoside 3'-phosphotransferase, Simian virus 40 SV40 pA, mouse Aqp4 genomic DNA
Research applicationCre/loxP system
FLP/frt system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Neurosci. Res., 64, 208-212 (2009).
Depositor照光（辻田）　実加（国立大学法人新潟大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Sep 2017

BRC mice in Publications

No Data