Strain Data Sheet

RBRC10015

Strain Information

Image
BRC No.RBRC10015
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Bcl6<tm1.1Toka>
Former Common nameYFP-Bc16
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain development岡田峰陽、2007-2009、理化学研究所免疫・アレルギー科学総合研究センターにて作成由来：B6N/B6J F1-hybrid ES主に兄妹交配（F30）、B6Jへの戻し交配５回（N5）SPF環境でのホモ接合体での致死性なし、発育良好、雌雄とも繁殖に問題なし。ホモ接合体では、抗体産生応答、とくに胚中心反応の減弱あり。
Strain descriptionThe monomeric yellow fluorescent protein (YFP) gene was inserted in-frame right after the initiation codon of the Bcl6 gene such that the product of this allele would be the YFP-Bcl6 fusion protein. Bcl6 protein expression can be analyzed by flow cytometry. Fluorescence of YFP-Bcl6 in most most cell types is too dim to be analyzed by microscopy unless cells/tissues are stained with anti-GFP and a fluorescent dye-labeled secondary antibody. YFP-Bcl6 is functionally compromised, possibly because fusion with YFP near the BTB domain of Bcl6 might impair recruitment of the corepressor molecules or disrupt the formation of Bcl6 homodimers or heterodimers with other BTB domain-containing transcription factors. Therefore, homozygous animals are hypomorphic. Heterozygous animals are largely normal.
Colony maintenance
ReferencesImmunity, 34(6), 961-972 (2011). 21636294

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bcl6B cell leukemia/lymphoma 616Bcl6<tm1.1Toka>targeted mutation 1.1, Takaharu Okada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)16EYFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Ordering Information

供与核酸phage P1 LoxP site, Aequorea victoria GFP variant(EYFP)cDNA, mouse Bcl6 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Immunity, 34(6), 961-972 (2011).
営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
Depositor岡田　峰陽（国立研究開発法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Jan 2019

BRC mice in Publications

No Data