Strain Data Sheet

RBRC09993

Strain Information

Image
BRC No.RBRC09993
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Fer1l5<tm1Osb>/95
Former Common nameFer1l5<+/-> #95
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeveloped by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed to BDF1. Mixed genetic background.
Strain descriptionFer1l5 knockout mice. Exon 2 and 3 were deleted.
Colony maintenance
References
Testis-enriched ferlin, FER1L5, is required for Ca2+-activated acrosome reaction and male fertility.
Akane Morohoshi, Haruhiko Miyata, Keizo Tokuhiro, Rie Iida-Norita, Taichi Noda, Yoshitaka Fujihara, Masahito Ikawa
Sci. Adv., 9(4):eade7607 (2023). 36696506

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fer1l5
MGI:3616091		fer-1 like family member 51Fer1l5<tm1Osb> targeted mutation 1, Research Institute for Microbial Diseases, Osaka UniversityNo MGI allelic designation

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAchicken beta-actin promoter, rabbit　beta-globin polyadenylation signal, CMV CMV IE enhancer, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter, mouse Fer1l5 genomic DNA, SV40 polyadenylation site, mouse PGK promoter, E.coli. Neomysin phosphotransferase gene (neoR), Yeast FRT site, Phage P1 loxP site
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Adv. Jan 25;9(4):eade7607.(2023) doi: 10.1126/sciadv.ade7607. Epub 2023 Jan 25.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data