Strain Data Sheet

RBRC09988

Strain Information

Image
BRC No.RBRC09988
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2;B6-Plcz1<em4(H435P)Osb>
Former Common namePlcz1<H435P/+>
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeveloped by Kaori Nozawa and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2015. Mixed genetic background. EGR-G101 ES cell was used. Mice were crossed with B6D2.
Strain descriptionPlcz1 point mutant (H435P) mice generated by the CRISPR/Cas9 technique. Plcz1<em4(H435P)Osb>: point mutation (H435P) at exon 10gaaagatgctgcgccagaggactgggagctaagccagataactcaagggttcctttaagtctgtgaaatgatcactaaattaatttaactagaaacctgtagcttcatatgacttacatgcgagtgcatcttaatgtgaaaattatgattaaaaattggtgaatatgttacttaaaatgtttaattttggaattgtgtgtaatcttgtattaacttggaattaatttggaagtgtgatcgtgaaatagttcaagtaaatgatcagggtacttaagttgctccatgaaataattataaaattttctgaattattttgtagcataagtgcataggttctttttttattttgtgattgataaatattcttgccaaggtcatgtatagaattattttacatatgtgagtaataatataaaaatatcttgtttcgctgactacaaatgtttcaaacattttcatttcttttttctctttgtagTCCATGAGTTTATTTTCCACACCGCGG(CCTACTGCAG)CATTCATCACCAGAGTATACCCCAAAATGATGAGAGCAGACTCTTCTAACTTTAACCCTCAAGAGTTTTGGAATGTAGGATGTCAGATGGgtatgttatgcagggtagtgacctatctgcaaatgtaagtagaaatgaattctttgtgtgagttaaaggacatttccagaaaagttttggccctggaagcaggatcaagcactcatggtaaaccactggtgtctagagctaaatttttcatcaaatagttggagagggcagatgtagttgaaggttggaaagcctagactaatattccaatatcctgatcttgtgaattagtctagactttccaaggtctggatattggaatatgtgcatttgagggagagggatactggatggatagatcgagttccttgcttgagtcaggtccaagtcttcttcaagccaagtgcccttcatcattttgaactaatatgagctttgacgttcacagtctcagaaggtgagtagggtcacagggctggttatttctccatgccagattctccttgatgcta
Colony maintenance
References
Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice.
Nozawa K, Satouh Y, Fujimoto T, Oji A, Ikawa M
Sci. Rep., 8(1):1315 (2018). 29358633

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Plcz1
MGI:2150308		phospholipase C, zeta 16Plcz1<em4(H435P)Osb> endonuclease-mediated mutation 4, Research Institute for Microbial Diseases, Osaka University
  • spermatogenic failure 17(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal calcium ion homeostasis(MP:0004231)

  • abnormal sperm physiology(MP:0004543)

  • decreased litter size(MP:0001935)

  • delayed blastocyst hatching from the zona pellucida(MP:0003695)

  • impaired fertilization(MP:0000242)
    • more 3 phenotypes
  • reduced fertility(MP:0001921)

  • reduced male fertility(MP:0001922)

  • reproductive system phenotype(MP:0005389)
    • Detailed phenotype data

    Ordering Information

    Donor DNAHuman hU6 promoter, CMV enhancer (CBh), Chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Streptomyces alboniger puromycin resistant gene, Escherichia coli Lac Z , T7 phage T7 promoter, Escherichia coli lac promoter, Mouse a part of Plcz1 gene
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep. 2018 Jan 22;8:1315. doi:10.1038/s41598-018-19497-6 RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data