Strain Data Sheet

RBRC09978

Strain Information

Image
BRC No.RBRC09978
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameSTOCK Gm17677<em1Osb>
Former Common namePateN<-294/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Taichi Noda and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2015. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed with B6D2F1. Mixed genetic background of B6D2F1 and 129S2.
Strain descriptionPateN (Gm17677) mutant mice generated by the CRISPR/Cas9 technique. 294 bp deletion in exon 1 and intron 1.Gm17677<em1Osb>: 294 bp insertion in exon 1 and intron 1.cccttgaacctgggattgtttcttgccaatgttattttcctttcaacatcccagtttgctaaaagggtgagaggaagcagaacacccactctccacaggaagagcctaccagcaataaatgggcctcattgttcagagtccatgagatcactcacagagtgggaggccaagaaaaagtcccagccccagctccatttcacatcctgcccagagcacagttcctgagcacttgttgaatactgagtctatatctcatccatcttttctttattgttttacccaaacacATGGACTGGCTACTGTTTCTCTTGCTTCCAGGAGTACTGATTTTGTACAAGTCTCATTCAATGGgtgagtgtaatggcagggaaacaaaggaagcagagctcttgggtatgggagtgatgtccccagcatcatgattggaaagggcaagagctacaagaacaacattagatccatgtctccaaattttctggctgtcataatcccatttctcaccttgcaagattttcctgggcatcttgatgtccaataacttcactagaatctagttctagggaggtattgtgatagactcaggggggagttaggtggggagggtgatacaaa
Colony maintenance
ReferencesBiol. Reprod. 2024 Jan 13:ioae008. 38217862

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gm17677predicted gene, 176779Gm17677<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Ordering Information

Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Mouse a part of Gm17677 (PateN) gene, rabbit beta-globin polyadenylation signal, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod. 2024 Jan 13:ioae008.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data