RBRC09977

Information1

Image
BRC No.RBRC09977
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameSTOCK Tcte1<em1(Tcte1-Flag)Osb>
Former Common nameTcte1<Flag/Flag>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 114(27):E5370-E5378 (2017). 28630322

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tcte1t-complex-associated testis expressed 117Tcte1<em1(Tcte1-Flag)Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Information2

Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Escherichia coli Lac Z, T7 phage T7 promoter, Escherichia coli lac promoter, Mouse a part of Tcte1 gene, synthetic Flag tag
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):E5370-E5378. doi: 10.1073/pnas.1621279114. Epub 2017 Jun 19. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Category I MTA: CRISPR/Cas9 genome edited bioresources ( Japanese / English )
Lab HP

BRC mice in Publications

No Data