BRC No.RBRC09976
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Gt(ROSA)26Sor<em1(CAG-3XFLAG/dCas9,-EGFP)Osb>
Former Common name3×FLAG-dCas9 KI
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Strain development
Strain description
Colony maintenance
ReferencesGenes Cells. 23(4):318-325 (2018). 29480524

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<em1(CAG-dcas9,-EGFP)Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University


Donor DNAHuman hU6 promoter, CMV enhancer (CBh), Chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Escherichia coli Lac Z , T7 phage T7 promoter, Escherichia coli lac promoter, Mouse a part of Gt(ROSA)26Sor, synthetic Flag tag, Streptococcus pyogenes dead Cas9 (dCas9), Mouse Rosa26, Rabbit rabbit beta-globin, EMCV Internal ribosomal entry site (IRES), SV40 polyadenylation site, jellyfish EGFP cDNA, SV40 NLS
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorDrs. Hodaka Fujii and Masahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Category I MTA: CRISPR/Cas9 genome edited bioresources ( Japanese / English )

BRC mice in Publications

No Data