Strain Data Sheet

RBRC09976

Strain Information

Image
BRC No.RBRC09976
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Gt(ROSA)26Sor<em1(CAG-3XFLAG/dCas9,-EGFP)Osb>
Former Common name3×FLAG-dCas9 KI
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain developmentDeveloped by Hodaka Fujii and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2015. EGR-101 ES cells derived from C57BL/6N were used and targeted at Rosa26 locus by the CRISPR/Cas9 technique. Mice were maintained in the B6D2F1 genetic background.
Strain description3×FLAG-dCas9-IRES-EGFP knock-in mouse at the Rosa26 locus.
Colony maintenance
References
Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis.
Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I, Fujii H
Genes Cells. 23(4):318-325 (2018). 29480524

Locus-Specific Genomic DNA Purification Using the CRISPR System: Methods and Applications.
Fujita H, Fujita T, Fujii H
CRISPR J., 4(2):290-300 (2021). 33876963

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<em1(CAG-dcas9,-EGFP)Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAHuman hU6 promoter, CMV enhancer (CBh), Chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Streptomyces alboniger puromycin resistant gene, Escherichia coli Lac Z , T7 phage T7 promoter, Escherichia coli lac promoter, Mouse a part of Gt(ROSA)26Sor, synthetic Flag tag, Streptococcus pyogenes dead Cas9 (dCas9), Mouse Rosa26, Rabbit rabbit beta-globin, EMCV Internal ribosomal entry site (IRES), SV40 polyadenylation site, jellyfish EGFP cDNA, SV40 NLS
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells. 2018 Apr;23(4):318-325. doi: 10.1111/gtc.12573. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorDrs. Hodaka Fujii and Masahito Ikawa (Hirosaki University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data