Strain Data Sheet

RBRC09961

Strain Information

Image
BRC No.RBRC09961
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Egfl6<em1Osb>
Former Common nameEgfl6<+1/Y>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Daiji Kiyozumi and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2015. Fertilized eggs derived from BDF1×BDF1 were used. Mixed genetic background.
Strain descriptionEgfl6 mutant mice generated by the CRISPR/Cas9 technique. 1 bp insertion at exon 2.Egfl6<em1Osb>: 1 bp insertion at exon 2.ctaactcagaattctttatccacagTCCATGGGATTATGGGTTGTCAGCACTGGCACACCAGCCTGGGGTCTGTCA(A)GTATGGAACTAAGATGGCCTGCTGCTATGGCTGGAAAAGGAACAACAAGGGAGTATGCGAAGgtaagttttgtaaacgtttagaccaccctgccattggatcaggatggccagttgtcaatctatgacaccattgtcaatagattctctggagtgaggacatgggtaactaccaatactccacaaagttttggtccccgtgcagtgtccagagtggttgacacttcac
Colony maintenance
References
CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice.
Abbasi F, Kodani M, Emori C, Kiyozumi D, Mori M, Fujihara Y, Ikawa M
Cells, 9(4):821 (2020). 32231122

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Egfl6
MGI:1858599		EGF-like-domain, multiple 6XEgfl6<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Mouse a part of Egfl6 gene, human hU6 promoter, CMV enhancer (CBh)
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cells. 2020 Mar 28;9(4). pii: E821.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data