Strain Data Sheet
RBRC09960
Strain Information | |
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| Image | |
| BRC No. | RBRC09960 |
| Type | Targeted Mutation and Transgene |
| Species | Mus musculus |
| Strain name | STOCK Adam3<tm1a(KOMP)Osb>/11B Tg(Clgn-Adam3Flag)1Osb |
| Former Common name | Adam3-Tg#2 |
| H-2 Haplotype | |
| ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Transgene was injected to fertilized eggs of BDF1 x heterozygous Adam3 KO. Mice were backcrossed to BDF1 for several generations. Mixed genetic background. |
| Strain description | Adam3 knockout mice with a transgene containing Flag-tagged Adam3 under the control of Clgn promoter. |
| Colony maintenance | |
| References | J. Cell Sci., 131(19) (2018). pii: jcs221481. doi: 10.1242/jcs.221481. 30209135 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Adam3a disintegrin and metallopeptidase domain 3 (cyritestin)8Adam3<tm1a(KOMP)Osb>targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Adam3a disintegrin and metallopeptidase domain 3 (cyritestin)UNAdam3mouse Clgn promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)UNFLAG |
Ordering Information | |
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| Donor DNA | chicken beta-actin promoter, rabbit beta-globin polyadenylation signal, CMV CMV IE enhancer, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter, mouse Adam3 genomic DNA, mouse En2 splice acceptor (En2 intron2/exon 3 splice acceptor), EMCV Internal ribosomal entry site (IRES), E.coli. lacZ, SV40 polyadenylation site, human Beta-actin promoter, E.coli. Neomysin phosphotransferase gene (neoR),Yeast FRT site, Phage P1 loxP site, mouse Calmegin promoter, mouse Adam3 cDNA, synthetic Flag tag |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Cell Sci. 2018 Oct 8;131(19). pii: jcs221481. doi: 10.1242/jcs.221481.Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |