Strain Data Sheet

RBRC09958

Strain Information

Image
BRC No.RBRC09958
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-4930451I11Rik<em1Osb>
Former Common name4930451I11Rik<-1/wt>, FIMP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. Fertilized eggs derived from BDF1×BDF1 were used. Mixed genetic background.
Strain description4930451I11Rik mutant mice generated by the CRISPR/Cas9 technique. 1 bp deletion at exon 2.4930451I11Rik<em1Osb>: 1 bp deletion at exon 2.ggcccagccccaggccttagtttccccaccccccccttcctttccccttctttcttccttttctcttttttccttcttcctcccttttgtctcattttactttgtcttgagacaaagtgttgctctatagcccaaaccttcagtccaaacctttcccccccatctcagacatggtgggcttctgatgggagctcagccagccctgggacaaaacaatcagagctctgggtttgcctggcctcattggtgggcatgatggcccttgggctcttgtcacagactctgctttggtttttagCCCCCAGAAGAGATGGAACCCGGCCCTCGGTTTCAGGAGCCAGACCCCAGCAAGTTGTAAACAGGCTTTTCTTTGATTATCCAGACTCAGACCGAGCTAGCCTTCTTGCTGTGGCCCGGTTTATTGGAGAGAAGCCTATAACGTTCGTTAAGACAGgtatgaatcagcttgatagaggggtcttcctcctggcggggtctggaaggacgctgttgttcattgtctggcagATTCCAGTCCTGGGCTCTTCCAGAACATCCTGGTGGGGACATTAGTGGTGGCCTTCTTCTTCCTGCTTTTCCAGTTCTGCTTGCATGTgtgagttgtgtctctgtgtcctcgccctaccccttccagaggacccaagtctcctatgtgtccctcctcatctgatctcc
Colony maintenance
References
Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail to fuse with oocytes in mice.
Fujihara Y, Lu Y, Noda T, Oji A, Larasati T, Kojima-Kita K, Yu Z, Matzuk R M, Matzuk M M, Ikawa M
Proc. Natl. Acad. Sci. USA, 117(17):9393-9400 (2020). 32295885

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
4930451I11Rik
MGI:1925368		RIKEN cDNA 4930451I11 gene74930451I11Rik<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Mouse a part of 4930451I11Rik gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A,117(17):9393-9400 (2020). doi: 10.1073/pnas.1917060117.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP, Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data