Strain Information | |
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Image | |
BRC No. | RBRC09952 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6D2-4930568D16Rik<em1Osb> |
Former Common name | 4930568D16Rik<-10/-10> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2013. Fertilized eggs derived from BDF1×BDF1 were used. Mixed genetic background. |
Strain description | 4930568D16Rik mutant mice generated by the CRISPR/Cas9 technique. 4 bp deletion at exon 2 and 6 bp deletion at intron 3.4930568D16Rik<em1Osb>: 4 bp deletion at exon 2 and 6 bp deletion at intron 3.caagacctgcttttgattgatgcctgccaaaacccagtttgtgtgctgatatctactattgcccctgtatctcagtgcattagtgggtaatgcctttgtatacctatgagacagtgtatatattttgaggctatcagttctgaggtctttaagtcaatgactggctttactatctttgtgatacactttgtttgactgtagggtaatctgaaatcagacactgaagttggtggcccaatttctacagattcattgaagacacctaagaaagtatttttaacctttaaccgaaatttttcctccctccctctctcctccactccctctctctagGAAGGAATGCAGTATAAAAAAGAAGCACTGTTGCTAATGCTGTTTGCTGTATTGCTGGCACTAACTCAACGCTTCTCCTACTCCAGgtaactatgtaaccctgaggtatcacagtcgctgattgacacagctgacctaaaatgctgggcttttccacaaaggcatcaaagcactctccaatggcaatggtgaacatatacctcagcgctttcactttttttcaactgtcaagaagtactgatcaaattcttggttctaggtaagcctcgtgccagctggtgccgagccacacagtgactcttgtcttagggagctttgcattctaggtggaaagaaacagcctgtttcctgtgc |
Colony maintenance | |
References | Biol. Reprod., 103(2):195-204 (2020). doi: 10.1093/biolre/ioaa084. 32561905 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter 4930568D16RikRIKEN cDNA 4930568D16 gene24930568D16Rik<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University |
Ordering Information | |
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Donor DNA | human hU6 promote, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coliampicillin resistant gene, Mouse a part of 4930568D16Rik gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 103(2):195-204 (2020). doi: 10.1093/biolre/ioaa084.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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No Data |