Strain Data Sheet

RBRC09921

Strain Information

Image
BRC No.RBRC09921
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Trp53<em1Rbrc>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentRIKEN BRC developed this strain by the CRISPR/Cas9 technique using fertilized eggs derived from C57BL/6NJcl. This was funded through the FY2016 AMED-NBRP fundamental technology upgrading program.
Strain descriptionTrp53 flox mice in the C57BL/6N genetic background. A pair of loxP sites flanks a genomic region from Exon 2 to Exon 9 of the Trp53 gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/03/11Room:3-3Rack:B
2023/12/11Room:3-3Rack:B
2023/09/11Room:3-3Rack:B
2023/06/13Room:3-3Rack:B
2023/03/13Room:3-3Rack:B
2022/12/12Room:3-3Rack:B
2022/09/12Room:3-3Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Trp53transformation related protein 5311Trp53<em1Rbrc>endonuclease mediated mutation 1, RIKEN BRC

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Ordering Information

供与核酸Phage P1 loxP sites, mouse Trp53 genomic DNA
Research applicationCre/loxP system
提供条件条件を付加する。
1, 研究成果の公表にあたってRIKEN BRCへの謝辞の表明を必要とする。
2, 営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
3, 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
DepositorRIKEN BRC（国立研究開発法人理化学研究所）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを3~6ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Enomoto Y, Katsura H, Fujimura T, Ogata A, Baba S, Yamaoka A, Kihara M, Abe T, Nishimura O, Kadota M, Hazama D, Tanaka Y, Maniwa Y, Nagano T, Morimoto M.
Autocrine TGF-β-positive feedback in profibrotic AT2-lineage cells plays a crucial role in non-inflammatory lung fibrogenesis.
Nat Commun 14(1) 4956(2023) 37653024