RBRC09911

Information1

Image
BRC No.RBRC09911
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Sik3<em2Iiis>
Former Common nameSik3<Flag>
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentWe induced random point mutations by ethylnitrosourea (ENU) and screened for dominant sleep and wakefulness abnormalities. Through our screening, we established a mutant pedigree, which we termed Sleepy (Slp), with a markedly prolonged sleep time. Whole-exome sequencing identified a heterozygous single nucleotide substitution at the splice donor site for intron 13 of the Sik3 gene. Further examine this function, we created the mice in which a Flag-tag was inserted into the N terminus of SIK3 (wildtype) using CRISPR/Cas9 technology.
Strain description
Colony maintenance
ReferencesNature, 539, 378-383 (2016). 27806374

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sik3SIK family kinase 39Sik3<em2Iiis>endonuclease-mediated mutation 2, Masashi Yanagisawa and Hiromasa Funato

Gene symbolGene nameChr.Allele symbolAllele namePromoter
FLAGFLAG tag (synthetic)9FLAG

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence9HA

Information2

Donor DNAmouse Sik3 genomic DNA, Synthetic FLAG tag, Human Influenza HA tag (Hemagglutinin gene)
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 539, 378-383 (2016).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorDrs. Hiromasa Funato and Masashi Yanagisawa (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Mar 2019
Category I MTA: CRISPR/Cas9 genome edited bioresources ( Japanese / English )

BRC mice in Publications

No Data