Strain Data Sheet

RBRC09911

Strain Information

Image
BRC No.RBRC09911
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Sik3<em2Iiis>
Former Common nameSik3<Flag>
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentDeveloped by Hiromasa Funato and Masashi Yanagisawa, University of Tsukuba. C57BL/6N background.
Strain descriptionMouse line harboring a Flag-tag inserted into the N terminus of SIK3 (wildtype) by the CRISPR/Cas9 technology. This line exhibits normal sleep and wakefulness and breeds well.
Colony maintenance
ReferencesNature, 539, 378-383 (2016). 27806374

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sik3SIK family kinase 39Sik3<em2Iiis>endonuclease-mediated mutation 2, Masashi Yanagisawa and Hiromasa Funato

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)9FLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence9HA

Ordering Information

Donor DNAmouse Sik3 genomic DNA, Synthetic FLAG tag, Human Influenza HA tag (Hemagglutinin gene)
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 539, 378-383 (2016).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from　Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorDrs. Hiromasa Funato and Masashi Yanagisawa (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Mar 2019

BRC mice in Publications

No Data