Strain Data Sheet

RBRC09905

Strain Information

Image
BRC No.RBRC09905
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Anxa10<em1Siag>
Former Common nameAnxa10 -/-
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentSHINJI SASAKI、2016
Strain descriptionB6D2受精卵の前核にCRISPR/CAS9でAnxa10(NM_001136089)の69-71bpのPAM、72-91bp標的配列として、74-81 bp (Axa10のexon2に位置) の8 bpの欠損したマウス。
Colony maintenance
ReferencesBMC Genomics, 17, 968- (2016). 27881083

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Anxa10annexin A108Anxa10<em1Siag>endonuclease-mediated mutation 1, Shinji Sasaki

Ordering Information

供与核酸pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine betaGH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]
Research application
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。BMC Genomics, 17, 968- (2016).
Depositor杉本　喜憲（公益社団法人畜産技術協会）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data