Strain Data Sheet

RBRC09881

Strain Information

Image
BRC No.RBRC09881
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Tmem173<em1Tsse>
Former Common nameSTING KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development北海道大学大学院医学研究科 高島 謙、押海 裕之(現：熊本大)、瀬谷　司、2015年大阪大学微生物病研究所付属遺伝情報実験センター内 特定非営利活動法人 発生工学研究会との共同研究により作出を行った。CRISPR/Cas9システムによる遺伝子編集により、ファウンダー世代においてTmem173 5塩基欠損変異マウスを得た。その変異マウスをC57BL6 WTマウスと交配して得られたヘテロ接合体マウス同士の交配により、5塩基欠損変異ホモ接合体マウスの系統を樹立した。
Strain descriptionSTINGはER上に存在する膜貫通タンパク質であり、細胞質内DNA認識経路のアダプター分子としてウイルス感染などに対するtype1 IFNの産生に重要な役割を果たす。dsDNAやSTINGリガンドである2’-3’-cGAMPによる刺激への応答性が消失する。
Colony maintenance
References
STING in tumor and host cells cooperatively work for NK cell-mediated tumor growth retardation.
Takashima K, Takeda Y, Oshiumi H, Shime H, Okabe M, Ikawa M, Matsumoto M, Seya T
Biochem. Biophy. Res. Comm., 478, 1764-1771 (2016). 27608599

Health Report

Examination Date / Room / Rack2024/10/07Room:3-BRack:J
2024/07/08Room:3-BRack:J
2024/04/08Room:3-BRack:J
2024/01/09Room:3-BRack:J
2023/10/10Room:3-BRack:J
2023/07/10Room:3-BRack:J
2023/04/10Room:3-BRack:J
2023/01/10Room:3-BRack:J

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tmem173transmembrane protein 17318Tmem173endonuclease-mediated mutation 1, Tsukasa Seya
  • STING-associated vasculopathy with onset in infancy(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased NK T cell number(MP:0008040)

  • decreased double-positive T cell number(MP:0005092)

  • decreased interferon-alpha secretion(MP:0008563)

  • decreased interferon-beta secretion(MP:0008565)

  • decreased interferon-gamma secretion(MP:0008567)
    • more 1 phenotypes
  • increased tumor growth/size(MP:0003721)
    • Detailed phenotype data

    Ordering Information

    供与核酸pX330-U6-Chimeric_BB-CBh-hSpCas9(Addgene plasmid #42230)[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9* (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR)**, E. coli pUC origin] * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan).** E. coli Ampicillin resistance gene was not detected by PCR. Other introduced genes were not tested.
    Research application
    提供条件利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。
    Depositor瀬谷　司, 高島　謙（国立大学法人北海道大学）
    Strain Status生体のアイコン生体
    凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結精子を1ヶ月以内に提供可能
    生体マウスを3~6ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Maekawa H, Inoue T, Ouchi H, Jao TM, Inoue R, Nishi H, Fujii R, Ishidate F, Tanaka T, Tanaka Y, Hirokawa N, Nangaku M, Inagi R.
    Mitochondrial Damage Causes Inflammation via cGAS-STING Signaling in Acute Kidney Injury.
    Cell Rep 29(5) 1261-1273.e6(2019) 31665638
    Suzuki Y, Sato T, Sugimori M, Kanemaru Y, Onodera S, Tsuchiya H, Nakamori Y, Tsuyuki S, Ikeda A, Ikeda R, Goda Y, Kaneko H, Irie K, Sue S, Maeda S.
    Activation of STING in pancreatic cancer-associated fibroblasts exerts an antitumor effect by enhancing tumor immunity.
    Sci Rep 14(1) 17071(2024) 39048609