Strain Information | |
---|---|
Image | |
BRC No. | RBRC09881 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | C57BL/6-Tmem173<em1Tsse> |
Former Common name | STING KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Ken Takashima, Hiroyuki Oshiumi and Tsukasa Seya, Hokkaido University in 2015. C57BL/6 genetic background. |
Strain description | Tmem173 (transmembrane protein 173) mutants generated by the CRISPR/Cas9 technique. 5 bp deletion was introduced at exon 3.Tmem173<em1Tsse>: 5 bp deletion at exon 3.ATGGGACTAGCTGGGCTGGGAGACCCAAGgagatggatgactagtcaggaccttaggccctgaaggacaggagaaccccagaagcatagctgtggatttcttgatgtctacagatgccatactccaacctgcatccagccatcccacggcccagaggtcaccgctccaaatatgtagccctcatctttctggtggccagcctgatgatcctttgggtggcaaaggatccaccaaatcacactctgaagtacctagcacttcacctagcCTCGCacgaacttggactactgttgaaaaacctctgctgtctggctgaagagctgtgccatgtccagtccaggtaaccctctgtggtctccacgatgacttgatacccagcaagtatcacacccttgactcctaaccttggccttccctgaacatcatcagctgagttgggctgggatctgaggctaggctgtcccctgcaggtaccagggcagctactggaaggctgtgcgcgcctgcctgggatgccccatccactgtatggctatgattctactatcgtcttatttctatttcctccaaaacactgctgacatatacctcagttggatgtttGGCCTTCTGGTCCTCTATAAGTCCCTAAGC |
Colony maintenance | |
References | Biochem. Biophy. Res. Comm., 478, 1764-1771 (2016). 27608599 |
Health Report | |
---|---|
Examination Date / Room / Rack | 2024/01/09Room:3-BRack:J 2023/10/10Room:3-BRack:J 2023/07/10Room:3-BRack:J 2023/04/10Room:3-BRack:J 2023/01/10Room:3-BRack:J 2022/10/11Room:3-BRack:J 2022/07/11Room:3-BRack:J |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter |
Ordering Information | |
---|---|
Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9(Addgene plasmid #42230)[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9* (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR)**, E. coli pUC origin] * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan).** E. coli Ampicillin resistance gene was not detected by PCR. Other introduced genes were not tested. |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Tsukasa Seya (Hokkaido University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
Maekawa H, Inoue T, Ouchi H, Jao TM, Inoue R, Nishi H, Fujii R, Ishidate F, Tanaka T, Tanaka Y, Hirokawa N, Nangaku M, Inagi R. Mitochondrial Damage Causes Inflammation via cGAS-STING Signaling in Acute Kidney Injury. Cell Rep 29(5) 1261-1273.e6(2019) 31665638 |